Association of methylation status of RASSFIA and APC promoters with prostate cancer / 第二军医大学学报

ABSTRACT

Objective To investigate the promoter methylation status of Ras association domain family 1 isoform A (RASSFIA) and adenomatosis polyposis coli promoter (APC) and its association with prostate cancer (PCa), so as to pave a way for early diagnosis of Pea. Methods The prostatie tissues and clinical data were collected from 60 patients with prostate cancer and 40 patients with benign prostatie hyperplasia (BPH) tissues. Bisulphite Sequencing PCR was used to detect the promoter CpG methylation of RASSFIA and APC genes. Results The methylation rates of promoter CG sites of RASSFIA and APC genes in Pea group were significantly higher whan those in the BPH group (RASSFIA 60. 8% vs 14%; APC 48. 84% vs 1. 19%, P<0. 05). The methylation rates in RASSFIA and APC genes were significantly associated with PSA, Gleason score, pathologic stage and risk classification of PCa (P< 0.01). Combined detection of methylation status of RASSFIA and APC genes yielded a sensitivity of 95. 74% and a specificity of 82. 90% in differential diagnosis of PCa and BPH. Conclusion The promoter methylation of RASSFIA and APC genes are associated with the development and progression of PCa, and the changes of methylation rate is closely associated with risk classification of PCa. Combined detection of methylation in RASSFIA and APC genes may be a promising method for early diagnosis of prostate carcinoma.