Influence of scopolamine hydrobromide orally disintegrating tablets on neurological behaviors in mice / 第二军医大学学报

ABSTRACT

Objective To study the effects of scopolamine hydrobromide orally disintegrating tablets (DTs) based nanospheres-particles (NiMS) system on the neurological behaviors in mice, so as to provide evidence for clinical administration. Methods The 3 tests in this study were all divided as following twenty male and 20 female mice were each randomly divided into 4 groups, with 5 mice in each group. Then a female and a male group were merged into one group, so there were 4 groups, each containing 10 mice. The four groups were subjected to the following treatments control, high dose (24 mg/kg), moderate dose (15 mg/kg) and low dose DTs (6 mg/kg). To study the effects of DTs on the balance capacity, we selected 40 mice with the ability to climb the bar from 50 mice. (1) The spontaneous activities of mice were observed and recorded 20 min after intragastric administration of DTs in each group. (2) Pentobarbital sodium was intraperitoneally injected in mice 20 min after intragastric administration of DTs to observe the sleep of mice, so as to evaluate the synergistic effect between the two drugs. (3) Mice were put on the top of a stainless bar 20 min after intragastric administration of DTs and the time spent by the mice from top to the bottom of bar was recorded to evaluate the impact of the medication on mouse balance capacity. Results (1) DTs at each dose had no significant influence on the spontaneous activity of mice compared with the controls (P>0. 05). (2) The sleep time of mice increased with the dose of DTs, and there were significant difference between control group with high dose and moderate dose groups (P<0. 05). (3) DTs shortened the time spent by mice from top to the bottom of the bar compared with the control group, with significant differences found in female mice taking three doses of DTs (P< 0. 01) and in male mice taking high and moderate dose of DT (P<0. 01, P < 0. 05). Conclusion Intragastric administration of DTs has no influence on spontaneous activity of mice, and its synergistic effect with pentobarbital sodium increases with dose. DTs can also decrease the balancing capacity of mice.