Crystal structure of Vibrio cholerae HutX protein / 第二军医大学学报

ABSTRACT

Objective To obtain purified Vibrio cholerae HutX and its diffraction data. Methods Protein HutX was obtained by gene cloning and protein expression, purified by nickcl sepharose affinity chromatography, anion exchange chromatography (source Q), and molecular sieve chromatography (Superdex 200), and identified by Western blotting analysis. Then the obtained protein was subjected to crystallization condition screening and hanging drop optimization. The obtained crystal structure was analyzed by X-ray diffraction method. Results Western blotting analysis indirectly indicated that the obtained protein was HutX protein. Then the HutX crystal and its diffraction data were obtained in the present study. Conclusion The findings of the present study pave a way for future research on the crystal structure and function of HutX protein and its role in heme utilization of Vibrio cholerae.