Effects of jinlida granules on renin-angiotensin system in kidney and cardiovascular tissues of diabetic rats / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1065-1069, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-839839
ABSTRACT
Objective To evaluate the effects of Jinlida granules on the renin-angiotensin system (RAS) in the kidney, heart and abdominal aorta of experimental diabetic rats, so as to explore the possible mechanism by which Jinlida granules protect the kidney and cardiovascular system. Methods The rats were randomized into 2 groups normal contronl group (CON group, n=10) and diabetic model group Cn = 20). Rats in the control group were fed with regular chow; those in the diabetic model group were fed with high-fat diet for 4 weeks, and then were administered with streptozotocin (STZ; 30 mg/kg) by intraperitoneal injection to induce diabetic model. Successful diabetic models were further randomized into two groups with 10 in each; Jinlida granules treatment group (DM + JLG group) and non-treatment group (DM group). Rats in DM + JLG group were given Jinlida granules orally (3 g/kg per day) for 8 weeks. Angiotensin I (Ang I) and angiotensin P (Ang II) were measured by enzyme-linked immunosorbent assay (ELISA) in the homogenates of the kidney, heart and abdominal aortas the protein expression of angiotensin II type 1 receptor (ATR) and angiotensin II type 2 receptor (AT2R) was detected by Western blotting analysis. Results Compared with CON group, the kidney mass/body mass(KM/BM), heart mass/body mass (HM/BM), blood glucose (BG), and 24-hour urine proteins (24 h UP) were significantly increased in the DM group (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Controlled clinical trial
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2012
Type:
Article
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