Relationship between single nucleotide polymorphism in genome of head and neck squamous cell carcinoma tissue and tumor metastasis / 第二军医大学学报

ABSTRACT

Objective: To screen for the genes associated with metastasis of head and neck squamous cell carcinoma (HNSCC) by studying the specimens of HNSCC. Methods: Eighty HNSCC specimens, including 17 involving the tongue, 13 involving mouth, 14 involving oropharynx, and 36 involving hypopharynx, were subjected to single nucleotide polymorphism chip analysis. The thirty-nine patients with distant metastasis during follow-up were taken as experimental group and the 41 without distant metastasis were taken as controls. Cox regression analysis was used to examine the influence of single nucleotide polymorphism (SNP) on distant metastasis of HNSCC. Results: The results of SNP demonstrated a distinct peak of frequent genomic gain at 11q13. Cox analysis of the array data showed the following results: a relative risk of T allele of rs965l738 as the SNP probe of SHANK2 gene was 3.61(P = 0.003), relative risk of T allele of rs4980690 as the SNP probe of FGF4 gene was 3.63(P = 0.006), relative risk of T allele of rs7929885 as the SNP probe of ANO1 gene was 4.35(P = 0.008), relative risk of T allele of rs687660 as the SNP probe of PPFIA1 gene was 4.24(P=0.011), relative risk of T allele of rs660665l as the SNP probe of ORAOV1 gene was 3.04(P = 0.013), and relative risk of T allele of rs592412 as the SNP probe of CCND1 gene was 4.07(P = 0.013). Conclusion: It is indicated that the following genes at 11q13 can increase the risk of metastasis of HNSCC, including T allele of rs965l738 in SHANK2, T allele of rs4980690 in FGF4, T allele of rs7929885 in ANO1, T allele of rs687660 in PPFIA1, T allele of rs6606651 in ORAOV1, and T allele of rs592412 in CCND1.