Anti-tumor constituents from the roots of Actinidia valvata / 第二军医大学学报

ABSTRACT

Objective: To study the anti-tumor constituents from the roots of Actinidia valvata Dunn. Methods: Column chromatographic techniques were used to isolate and purify the chemical constituents of the plant and their structures were elucidated by spectroscopic analysis, including 1HNMR, 13CNMR and MS. The cytotoxic activities of some individual compounds were examined by MTT assay. Results: Ten triterpenoids 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid(1), asiatic acid(2), corosolic acid(3), 2α, 3α, 23, 24-tetrahydroxyurs-12-en-28-oic acid(4), 2α, 3α, 13β, 24-tetrahydroxyurs-11-en-28-oic acid-13-lactone(5), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid(6), 2α, 3α, 19α, 24-tetrahydroxyurs-12-en-28-oic acid(7), 2α, 3α, 24-trihydroxyurs-12, 20(30)-dien-28-oic acid(8), 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid(9), and oleanolic acid (10), and one plant sterol β-citosterol(11) were isolated from the CHCl3 fraction of the roots of Actinidia valvata Dunn. The cytotoxic activities of five compounds (1, 2, 3, 4, and 7) against A549, LOVO and HepG2 cell lines were evaluated. Conclusion: Compounds 3-8 are isolated from this plant for the first time. Compounds 2 and 3 possess cytotoxic activity against LOVO and HepG2 cell lines, and the cytotoxic activity decreases with the increase of polarity of individual compound.