Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 607-611, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-840035
ABSTRACT
Objedive To observe the effect of microRNA-29c cm the proliferalion and invasion abililies of hirman prostate carreer cell litre LNCaP, and to discijss its potetrlial applicasion. Methods The difference in miR-29c expression between ADPC (androgen-dependent prostate cancer) and AIPC (androgen-independent prostate cancer)cell lines was observed by real time RT-PCR.MiR-29c-inhibitor (anti-miR-29c) was used to decrease miR-29 cexpression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c.Results The result of real-timeRT- PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells (P<0.05).Down- expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells (P<0.05). Conclusion Normal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells, in dicating it might be a new target for biotherapy of prostate cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2011
Type:
Article
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