Polymers poly (β-amino esters) nanoparticles for gene delivery / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 473-476, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-840049
ABSTRACT
Objective:
To synthesize a new type of cationic polymers (β-ammo esters) (PBAE) and to study its role as a gene vector.Methods:
PBAE was synthesized by the Michael addition reaction. PBAE/pDNA complex nanoparticles were prepared by self-assembly, and the gene tranfection efficiency mediated by the nanoparticles into HEK293 cells in vitro was observed.Results:
When weight ratio of PBAE/pDNA was 50 1, pDNA was completely wrapped. And the transfection efficiency of PBAE/pDNA nanoparticles (PBAE/pDNA weight ratio being 200 1) was significantly higher than PEI/pDNA nanoparticles (43.3% ± 3.7% vs 30.3% ± 2.1%, P < 00.05). Conclution Cationic poly(β-amino esters) can electrostatically bind to and condense anionic DNA into nanometersized parties. PBAE/pDNA nanopartides has a high transfection efficiency in in vitro transfection experiments.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2011
Type:
Article
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