Inhibitory effect of nitric oxide-donating aspirin against prostatic cancer angiogenesis in tumor-bearing mice / 第二军医大学学报

ABSTRACT

Objective: To study the inhibitory effect of nitric oxide-donating aspirin(NO-ASA) on prostatic cancer growth and angiogenesis in subcutaneously-injected tumor in mice. Methods: The tumor-bearing mice were subcutaneously injected with NO-ASA, and the tumor growth was observed. ELISA was used to assay the expression of VEGF in mouse sera; real-time PCR was used to examine the expression of VEGF mRNA in tumor tissues; immunohistochemistry was used to detect CD34 expression and to quantify the number of microvessels in the tumor; and Hen's egg test chorioallantoic membrane (HET-CAM) was employed to detect the in vitro angiogenesis. Results: NO-ASA inhibited the prostate cancer growth in mice. The expression of VEGF was greatly decreased in the serum and tumor tissues, and CD34 expression was significantly decreased in the tumor tissues compared with the control group (P<0.05, P<0.01). NO-ASA also greatly inhibited the angiogenesis in HET-CAM. Conclusion: NO-ASA can inhibit prostate cancer growth and angiogenesis.