Matrine, oxymatrine and sophordine inhibit secretion of TNF-α and expression of CD91 and CD13 in macrophage RAW264.7 / 第二军医大学学报

ABSTRACT

Objective: To observe the inhibitory effects of matrine, oxymatrine and sophordine on expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7. Methods: Macrophages were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0,12. 5,25,50,100,200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybemetic, Inc. , 6.0 edition). Meanwhile, the levels of TNF-α in the culture medium of macrophages were examined by ELISA. Results: Matrine, oxymatrine and sophordine could all inhibit CD91 and CD13 expression in macrophage RAW264.7, and the inhibition varied with the concentrations of matrine, oxymatrine and sophordine; the inhibitory effect of matrine was the most prominent one. Moreover, matrine, oxymatrine and sophordine also inhibited the secretion of TNF-α. Conclusion: Matrine, oxymatrine and sophordine can greatly inhibit expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7.