Comparison of tolerance to ischemia-reperfusion injuries between different parts of extrahepatic bile duct during orthotopic autologous liver transplantation in rats / 第二军医大学学报

ABSTRACT

Objective: To observe the tolerance to ischemia-reperfusion injuries in different parts of extrahepatic bile duct during orthotopic autologous liver transplantation in rats, so as to lay a foundation for selection of anastomosis site during liver transplantation. Methods: Thirty SD rats were randomly divided into 3 groups group I, sham operated(n=6); group II, 1 h ischemia in biliary tract followed by 1 h reperfusion (n=12); and group III, 1 h ischemia in biliary tract followed by 2 h reperfusion(n=12). TUNEL assay, pathomorphology score and ultrastructural quantitative analysis were used to analyze the epithelia of hilar bile duct, proximal, and distal common bile duct. Results: The results of TUNEL assay and pathomorphology score were not significantly different between the proximal and distal common bile ducts in group II (P>0.05), but the injury was more serious in hilar bile duct compared with those of the proximal and distal common bile ducts (P<005); the hilar bile duct had the most serious injuries as indicated by the changes of mitochondrial mean volume (V) and area density of microvilli (AMV), and the proximal common bile duct had the slightest injury (P<0.05). In group 1, the most severe injury was found in the hilar bile duct, followed by distal common bile duct and proximal common bile duct in order (P<0.05). Conclusion: The tolerance to ischemia-reperfusion is different in different parts of bile duct during liver transplantation, with the distal common duct having the best tolerance, which may be used for bile anastomosis during transplantation.