Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 783-785, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840530
ABSTRACT
Objective:
To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library.Methods:
The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph. D. -7 peptide library with phage display technique, and the positive clones were identified by ELISA.Results:
After three rounds of biopanning, the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA. Sequencing result showed that the binding peptides had high affinity and specificity.Conclusion:
A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library, which paves a way for the diagnosis and treatment of hepatitis B infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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