Interaction between ciprofloxacin and ferric-ion binding protein from pathogenic bacteria / 第二军医大学学报

ABSTRACT

Objective: To investigate the interaction between ciprofloxacin (CIP) and ferric-ion binding protein (FBP) from pathogenic bacteria, so as to determine whether FBP is the target of CIP. Methods: Reactions between ciprofloxacin and FBP from N. gonorrhoeae, which was expressed in E. coli, were monitored by UV-visible and NMR spectroscopy. Results: Fe3+ was removed from holo-FBP by CIP in 10 mmol/L Tris-Cl buffer, pH 7.40, at 298 K, and half of the loaded Fe3+ was taken off when 50 molar equivalence of CIP was present. Conclusion: CIP can remove Fe3+ from holo-FBP, indicating that CIP can interfere with the Fe3+ capture of the bacteria and influence the growth and virulence of the bacteria.