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Interaction between ciprofloxacin and ferric-ion binding protein from pathogenic bacteria / 第二军医大学学报
Academic Journal of Second Military Medical University ; (12): 808-811, 2010.
Article in Chinese | WPRIM | ID: wpr-840536
ABSTRACT

Objective:

To investigate the interaction between ciprofloxacin (CIP) and ferric-ion binding protein (FBP) from pathogenic bacteria, so as to determine whether FBP is the target of CIP.

Methods:

Reactions between ciprofloxacin and FBP from N. gonorrhoeae, which was expressed in E. coli, were monitored by UV-visible and NMR spectroscopy.

Results:

Fe3+ was removed from holo-FBP by CIP in 10 mmol/L Tris-Cl buffer, pH 7.40, at 298 K, and half of the loaded Fe3+ was taken off when 50 molar equivalence of CIP was present.

Conclusion:

CIP can remove Fe3+ from holo-FBP, indicating that CIP can interfere with the Fe3+ capture of the bacteria and influence the growth and virulence of the bacteria.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Academic Journal of Second Military Medical University Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Academic Journal of Second Military Medical University Year: 2010 Type: Article