Interaction between ciprofloxacin and ferric-ion binding protein from pathogenic bacteria / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 808-811, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840536
ABSTRACT
Objective:
To investigate the interaction between ciprofloxacin (CIP) and ferric-ion binding protein (FBP) from pathogenic bacteria, so as to determine whether FBP is the target of CIP.Methods:
Reactions between ciprofloxacin and FBP from N. gonorrhoeae, which was expressed in E. coli, were monitored by UV-visible and NMR spectroscopy.Results:
Fe3+ was removed from holo-FBP by CIP in 10 mmol/L Tris-Cl buffer, pH 7.40, at 298 K, and half of the loaded Fe3+ was taken off when 50 molar equivalence of CIP was present.Conclusion:
CIP can remove Fe3+ from holo-FBP, indicating that CIP can interfere with the Fe3+ capture of the bacteria and influence the growth and virulence of the bacteria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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