Protective effect of hyperpolarized preservation solution combining sodium-hydrogen exchange inhibitor and potassium channel opener on donor heart / 第二军医大学学报

ABSTRACT

Objective: To study whether sodium-hydrogen exchange inhibitor(NHEI) can enhance the protective effect of hyperpolarized preservation solution containing potassium channel opener (PCO), and the effect was compared with that of Celsior solution. Methods: After equilibrated on a crystalloid-perfused Langendorff model for 30 min, rabbit hearts were divided into four groups (1) Cel group receiving another 30 min of equilibration, 8 h cold storage with Celsior solution and 1 h reperfusion; (2) Pin group arrested and stored with pinacidil (a type of PCO) hyperpolarizing cardioplegia; (3) Cel+Car group receiving the same treatment as Cel group except that 10 μmol/L cariporide (a type of NHEI) was administered during the second equilibration period and reperfusion; and (4) Pin+Car group receiving cariporide treatment and hyperpolarized arrest and storage. Results: Recovery rate of systolic function in the Pin+Car group was significantly higher than that in the Pin group ([87.4±11.9]% vs [69.0±7.2]%, P0.05). No significant differences were found in any parameters between Cel group and Cel+Car group. There were no differences in coronary flow among all groups. Conclusion: NHEI can greatly enhance the protective effect of PCO hperpolarized preservation solution on the donor heart, and the combination of them has a comparable protective effect to Celsior solution.