Anti-apoptosis effect of tremella polysaccharides on cardiomyocytes / 第二军医大学学报

ABSTRACT

Objective: To observe the anti-apoptosis effect of tremella polysaccharides (TP) on cardiomyocytes by in vitro and in vivo experiments. Methods: TP was extracted by dehydrated alcohol and the TP purity was measured by gas chromatography. The cultured cardiomyocytes of the neonatal rats were randomly assigned to normal group (Group A), apoptosis-induced group (Group B) and TP-pretreatment group (Group T). The morphology and uptake rate of trypan blue were studied after 72 h. The apoptosis index of cardiomyocyte was measured by flow cytometry. In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks. Results: Data of cultured cardiomyocytes were as follows Uptake rate of trypan blue B>C>A (PC> A (P<0.01). In the animal studies there were no obvious difference in myocardium pathology between groups. The order of the apoptosis indices of myocardium was HMyocardium homogenate detection showed that MDA and LP in Group H, M were lower than those in group C (P<0.05); MDA and LP in group H were lower than those in group M (P<0.05); the activities of GSH-Px and SOD in all TP-pretreatment groups were higher than those in group C (P<0.05), and that in group H was the highest (P<0.05).

Conclusion:

TP can suppress the apoptosis of cardiomyocytes induced by oxidative damage in vitro, indicating a protective effect on cardiomyocytes. Our results suggest that TP has anti-apoptosis and anti-oxidation effect on cardiomyocytes in D-galactose-induced aging mice and the effect is dose related.