Relationship of hepatitis B virus genotypes with clinicopathological characteristics in patients with chronic hepatitis B and hepatocellular carcinoma / 第二军医大学学报

ABSTRACT

Objective: To investigate the distribution of different hepatitis B virus (HBV) genotypes in patients with chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC), and to analyze the clinical laboratory examination outcomes and pathological characteristics of CHB and HCC by infection with different HBV genotypes. Methods: Totally 89 patients with CHB and 86 patients with HCC were randomly chosen for this study. HBV genotypes were determined by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) combined with double immunofluorescence staining TaqMan MGB probes. The general information and the laboratory and pathological data of patients were obtained by reviewing of the clinical documentation of patients. Statistical software SPSS10.0 was used to for statistical analyses. A P value less than 0.05 was considered statistically significant. Results: HBV B was dominant in the CHB patients in our group, accounting for 78.65%; the mixed B and C type accounted for 3.37%. HCC patients in our group were dominated by C type (70.93%). There were no other genotypes other than B and C in our group, and there was significant different between their frequency in our group (P<0.001). There were no significant differences in the clinical experimental and pathological parameters in CHB patients with different HBV subtypes. In HCC patients, those with genotype C had higher positive rate of HBV e antigen than those with genotype B(P<0.05). HCC patients infected with HBV genotype B had larger turner size (P<0.05). No associations were found between HBV genotypes with TNM stage, vascular invasion, or metastasis. Conclusion: Patients with CHB are dominantly infected with genotype B in our group. HBV genotype C and positive HBV e antigen are risk factors of HCC. Antiviral therapy and promoting e antigen seroconversion may reduce the incidence of HCC. HBV genotype B might be associated with larger tumor size.