Effect of Cytochalasin B on Survivability and in Vitro Development of Mouse Oocyte Frozen by Vitrification / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 317-322, 2003.
Article
in Korean
| WPRIM
| ID: wpr-84065
ABSTRACT
OBJECTIVE:
The purpose of this study was to evaluate the effect of CCB treatment on the survivability and in vitro development of mouse oocyte frozen by vitrification.METHODS:
Mouse oocytes retrieved from cycle stimulated by PMSG and hCG were treated by CCB and then exposed to EFS-30. These oocytes were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing of the oocytes on EM grid was carried out at room temperature for 5 seconds, then the EM grid was placed into 0.75 M sucrose at 37degrees C for 3 minutes. This was followed by 0.5 M and 0.25 M sucrose for 3 minutes, each. We compared the survivability, cleavage and developmental rate of mouse frozen by vitrification between CCB treated and non-treated groups. Chi-square was used to determine statistical significance. statistical significance was defined as p<0.05.RESULTS:
Survivability (79.3%) and developmental rate into blastocyst (52.3%) of mouse oocyte were markedly decreased after vitrification. There were no significant differences between CCB treated and non- treated groups regarding survivability of oocyte frozen by vitrification (80.3% vs 78.5%). The developmental rate into 2-cell in CCB treated group was significantly higher than that in non-treated group (69.7% vs 61.9%, p<0.05). The developmental rate into blastocyst in CCB treated group was higher than that in non-treated group (54.9% vs 51.5%), but the difference was not significant.CONCLUSION:
Survivability of mouse oocyte could not be affected by CCB treatment and developmental rate into 2-cell was improved in CCB treated group. It is suggested that CCB treatment prior vitrification improve stability of cytoskeleton and then improve fertilization and early stage embryo development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Sucrose
/
Blastocyst
/
Cytoskeleton
/
Cytochalasin B
/
Embryonic Development
/
Fertilization
/
Vitrification
/
Nitrogen
Limits:
Animals
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS