Expression of peroxisome proliferators-activate receptor γ in renal tissue of aging rats and its relationship with oxidative stress / 第二军医大学学报

ABSTRACT

Objective: To investigate the expression of peroxisome proliferator-activate receptor γ(PPARγ) in the kidneys of rats at different ages, so as to study the possible association of PPARγ expression with oxidative stress in the aging process. Methods: Rat aging model was established using 3-, 12-, and 24-month old rats. Western blotting was used to determining the protein expression of PPARγ. The changes anti-oxidative ability of renal tissue were studied by determining the activities of superoxidedismutase(SOD) and glutatio-neperoxidase(GSH-PX). Results: Western blotting assay showed that the renal expression of PPARγ protein decreased with the aging of rats,with that of 3-month group significantly higher than that of the 24-month group(P<0.01). The activities of SOD and GSH-PX deceased with the aging of rats(P<0.01). Both the SOD activities were positively correlated with the expression of PPARγ protein(r=0.90, r=0.89, P<0.01) Conclusion: Renal PPARγ has a decreasing tendency during the aging process of rats, which is correlated with the down-regulation of anti-oxidative ability of renal tissue.