Establishment of androgen-independent human prostate cancer line LNCaP by gradual deprivation of hormone / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1311-1315, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840704
ABSTRACT
Objective:
To establish and identify androgen-independent human prostate cancer cell line LNCap by culturing LNCaP cells with gradual deprivation of hormone.Methods:
LNCaP cells were cultured in the medium with gradual deprivation of hormone (treated by active carbon to simulate androgen deprivation) for 10 days; and then the cells were cultured with complete deprivation of androgen for 3 months till the cell entered the rapid proliferation phase again. The cell growth and expression of PSA and androgen were examined by CCK-8, immunfluorescence and RT-PCR methods.Results:
LNCaP cells grew slowly after deprivation of hormone and took on a neuroendocrine phenotype and cluster growth pattern. After 3 months' non-androgen culture,the cells regained original morphology and growth. CCK-8 indicated that LNCaP cells could grow in non-androgen condition; immunofluorescence assay indicated that LNCaP-AI cells could regain PSA-secreting activity in non-androgen condition; and RT-PCR suggested that androgen was highly expressed in LNCaP-AI cells.Conclusion:
Androgen-independent LNCaP cell line can be established by culturing with gradual deprivation of hormone for 3 months.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS