Effects of simvastatin on renal expression of Fas/Fas-L in aged diabetic rats after uninephrectomy / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1046-1051, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840755
ABSTRACT
Objective:
To explore the effects of hydroxy-methyl-glutaryl coenzymeA reductase inhibitor (HMG-CoA RI) simvastatin on renal expression of apoptosis related gene Fas/Fas-L in aged diabetic rats after uninephrectomy.Methods:
Elderly uninephrectomized SD rats were randomly divided into uninephrectomized control group (group C), streptozocin(STZ)-induced diabetic model group(group D), and diabetes + simvastatin group (group S). The apoptosis indices in renal tisses of three groups were detected by terminal deoxynucleotide mediated nick end labeling 12 weeks later. Immunofluorescence and real-time quantitative PCR were employed to observe the expression changes of Fas/Fas-L genes at the transcription and translation levels.Results:
Compared with group C, group D had significantly higher apoptosis index and expression level of Fas/Fas-L; the above parameters were lower in group S compared with those in group D (P<0.05). The expression of Fas/Fas-L protein was mainly in the renal tubules.Conclusion:
Both Fas and Fas-L genes are associated with the development and progression of diabetic nephropathy in aged rats. Simvastatin can down-regulate the translation and transcription of the genes, inhibit cell apoptosis and protect the kidney.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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