Inhibitory effect of selective cyclooxygenase-2 inhibitor NS-398 on proliferation and invasion of human colorectal cancer cells / 第二军医大学学报

ABSTRACT

Objective: To investigate the effect of NS-398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation and invasion of human colon carcinoma cells in vitro, so as to determine the possibility of COX-2 as a new target for treatment of colon carcinoma. Methods: The expression of COX-2 in colorectal cancer cells (CW-2, COLO-320) was detected by RT-PCR and Western blotting. COLO-320 cell proliferation was measured by MTT after treatment with NS-398. Cell invasion ability was measured using migration and invasion chamber systems. Western blotting assay was used to examine the influence of NS-398 on MMP 2 expression. Results: Our results showed that CW-2, COLO-320 cells expressed COX-2 mRNA and protein. NS-398 inhibited the proliferation of COLO-320 cells in a time and concentration-dependent manner. Invasion test showed that NS-398 inhibited the migration and invasion of COLO-320 cells. Western blotting revealed that NS-398 inhibited the expression of MMP-2 in COLO-320 cells. Conclusion: The selective COX-2 inhibitor NS-398 can inhibit COLO-320 cell proliferation and invasion, indicating COX-2 may serve as a new target for colon carcinoma treatment.