Changes in common chemical signal molecules of neuroendocrine-immune network and intervention of Tongxinluo in rats under hypoxia qi-asthenia condition / 第二军医大学学报

ABSTRACT

Objective: To investigate the changes in common chemical signal molecules of neuroendocrine-immune (NEI) network and intervention of Tongxinluo (TXL, ultramicro-pulverization) of rats under hypoxia qi-asthenia condition. Methods: Forty-five male Wistar rats were randomly assigned to the following groups control group, qi-asthenia model group and TXL group (n=15). The rats in the former 2 groups were gavaged with CMC-Na (10 ml/ kg) once daily; those in the TXL group were gavaged with TXL (ultramicrot-pulverization) suspended in CMC-Na (10 ml/kg) once daily; After gavage, animals in the qi-asthenia model group and TXL group were placed in a cabin with normal pressure hypoxia (7 h daily, 6 d weekly for 5 weeks). At the end of the experiment, the biological characters of rats were observed and arterial blood gas was analyzed. The serum contents of serotonin (5-HT), norepinephrine (NE), epinephrine (E), dopamine (DA) and IFN-γ and TRH level in the hypothalamus were examined by enzyme linked immunosorbent assay (ELISA); the serum contents of corticosterone (Cort), T3,T4,IL-1β,TNF-α, and corticotropin release hormone(CRH) in the hypothalamus, and adrenocorticotropic hormone/thyroid stimulating hormone(ACTH/TSH) in the hypophysis cerebri were examined using radio-immunity method. Results: Compared with control group, the biological character scores of rats in the qi-asthenia model group were significantly bigher(Ppartial pressure of oxygen was significantly lower(P<0. 05) the contents of 5-HT,DA,NE and E were significantly decreased (Padrenergic nerve-adrenal medulla system was restrained and the release of IL-1β,IFN-γ and TNF-α was significantly increased(Pbiological character score and increase the arterial partial pressure of oxygen in model rats, raise 5-HT and DA levels, restrain HPA and HPT system excitation, and decrease IL-1β and IFN-γ levels.

Conclusion:

The qi-asthenia model can be successfully established using normal pressure chronic hypoxia. The NEI network regulation is chaotic and in a disequilibrium state, which might be an important basis for hypoxia qi-asthenia syndrome. TXL can enhance the hypoxia tolerant capability of model rats and improve their qi-asthenia syndrome, probably through adjusting NEI network disequilibrium.