Preparation of injectable sustained-release nanoparticles carrying brain-derived neurotrophic factor and evaluation of their drug releasing characteristics / 第二军医大学学报

ABSTRACT

Objective: To prepare stable, small-sized injectable sustained-release nanoparticles harboring brain-derived neurotrophic factor (BDNF) and to evaluate its drug releasing process. Methods: The nanoparticles were prepared using poly (D,L-lactic-co-glycolic acid) (PLGA) as the carrier by w/o/w double emulsion-solvent evaporation method. The formula and technique were optimized; the shape,size and the distribution of the diameters of the particles were observed; and recovery rate, precision, repeatability, encapsulation efficiency,and drug releasing characteristics were assessed. Results: With the optimized formula, the drug loading rate was 1%,the polymer concentration was 3.3 mg/ml, and the ultrasound time was 40 s; mannitol was used as the supporting agent. BDNF nanoparticles were round, homogenous in size, with a mean diameter of 156.7 nm. The prepared particles had high recovery rate, precision, repeatability, and encapsulation efficiency. The drug release was characterized by slow corrosion and the process lasted for 30 days. Conclusion: We have successfully prepared slow-release nanoparticles harboring BDNF, which are stable and have high encapsulation efficiency.