Influence of total saponins from Asparagus cochinchinensis on cerebral blood flow and vascular resistance in anesthetized dogs / 第二军医大学学报

ABSTRACT

Objective: To study the effect of total saponins on cerebral blood flow and vascular resistance in anesthetized dogs. Methods: Thirty hybrid dogs in either sex, with a body weight of (11±1.5) kg, were evenly randomized into 5 groups negative control group(saline 5 ml/kg,ig), positive control (nimodipine 300 μg/kg, iv), and 3 groups treated with total saponins (low-dose group [1O mg/kg, ig], middle-dose group[30 mg/kg, ig], and high-dose group[60 mg/kg, ig]). The dogs were anesthetized with intravenous pentobarbital sodium (30 mg/kg). The right common carotid artery was exposed to measure the cerebral blood flow, cerebral vascular resistance, blood pressure and heart rate using the MFV-3200 electromagnetic flow meter and MPA-3000 bioelectricity signal-amplifier. Results: Compared with negative control, cerebral blood flow was significantly increased in animals treated with asparagos root saponins (30 and 60 mg/kg, ig) during 5 and 120 min after drug administration (P<0.01). No significant effect on cerebral blood flow and vascular resistance was found in animals treated with asparagus root saponins (10 mg/kg). Conclusion: Asparagus root saponins can increase cerebral blood flow in anesthetized dogs.