Expression of heterogeneous nuclear ribonucleoprotein K in hepatocellular carcinoma tissues and cell line of different cell densities / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 20-23, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840957
ABSTRACT
Objective:
To investigate the expression changes and the subcellular localization of heterogeneous nuclear ribonucleoprotein K (hnRNP K) in hepatocellular carcinoma (HCC) and cell line of different cell densities.Methods:
The expression of hnRNP K in HCC was detected by immunohistochemical staining. The differential expression of hnRNP K in the HCC tissue (n = 30) and paired paracancerous tissue was identified by Western blotting assay. The expression and sub-cellular location of hnRNP K in HCC cell line of different cell densities were determined by immunocytochemical staining and Western blotting assay.Results:
Expression of hnRNP K was mainly detected in the nucleus of HCC cells. Twenty-one of the 30 hepatic cancer tissues had higher hnRNP K expression than the paired paracancerous tissues. Sixteen of the 20 HCC samples with HBV infection had an increased expression of hnRNP K; in the 10 HCC samples without HBV infection, 5 had an increased expression of hnRNP K. Immunocytochemical staining and Western blotting assay both showed that the expression of hnRNP K was in creased in the nucleus of HCC cells with the increase of cell densities (30%, 50% and 90% confluence); the result of Western blotting assay also showed that there was no obvious change in hnRNP K expression in the cytoplasm of HCC cells of different cell densities(30%, 50%, 70% and 90% confluence).Conclusion:
The expression of hnRNP K is elevated in HCC tissues and has an increasing tendency in the nucleus with the increase of cell density. Infection of HBV may be associated with the up-regulation of hnRNP K in HCC cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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