Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors / 第二军医大学学报

ABSTRACT

Objective: To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors. Methods: NV and TOE were separated by autolysis and centrifugation. The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed. Results: NV and TOE were successfully separated. The concentration-associated haemolytic curves were "S" shaped for both NV and TOE. The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE. Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40°C. pH value also had influence on haemolysis. The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive. Conclusion: NV and TOE both have haemolysis activity, with the activity of NV stronger than that of the latter. The haemolysis activity is influenced by concentration, temperature and pH value.