Analysis of polymorphism in human cDNA sequence of prothymosin-α / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 92-94, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-840972
ABSTRACT
Objective:
To analyze the polymorphism in human cDNA sequence of prothymosin α (ProTα) by sequencing analysis.Methods:
The cDNA of human ProTα was amplified from cells of peripheral blood and cord blood by RT-PCR. The product of RT-PCR was purified and linked with vector pMD18-T. After cloning and sequencing, the sequence of ProTα cDNA was compared with the standard sequence to analyze the polymorphism in the ProTα cDNA sequence.Results:
The cloned ProTα cDNA sequence was different from that of the standard. We found 2 kinds of variations (1) The nucleotide in 107 position was varied and the nucleotides in 110-121 and 191-205 positions were deleted; (2) The nucleotide in 306 position was deleted, mainly in the 60-80 years old group.Conclusion:
We have identified 2 kinds of variations in human ProTα cDNA, but the first 28 amino acid in the N-terminal of cDNA of human ProTα are not involved therefore the variations do not affect the function of human ProTα.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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