Vitamin E Succinate inhibits growth and induces apoptosis of prostate cancer cell line PC-3 / 第二军医大学学报

ABSTRACT

Objective: To study the inhibitory and apoptosis-inducing effect of vitamin E succinate (VES) on androgen-independent prostate cancer cell line PC-3 in vitro. Methods: VES was dissolved with ethanol to obtain VES solution. PC-3 cells of logarithmic growth phase were treated with various concentrations of VES solution (25, 50, 75, 100, and 125mg/L); cells in control group were treated with 1.25% ethanol. MTT method was used to measure the viability and inhibitory rate of cells in each group 24h, 48h and 72h after VES treatment; flow cytometry was employed to determine the apoptosis rate of the PC-3 cells. Results: The viability of cells in the experimental groups was significantly lower than that in the control group (P<0.05). The viability of cells in the experimental groups was negatively correlated with the concentration and exposure period of VES solution; the viability of cells in the control group was positively correlated with the exposure period of VES solution. The apoptosis rate of cells in the experimental groups was much higher than that in the control group; the rate in the experimental group was positively correlated with the concentration and exposure period of VES solution. The optimal induction of apoptosis was achieved af ter 48 h exposure to 75 mg/L VES solution, with a apoptosis rate above 80%. Conclusion: VES can inhibit the proliferation of PC-3 cells and can induce apoptosis of them, which casts new lights on prevention and treatment of prostate cancer.