Changes of TNF-α and IL-10 expression in transplanted laryngeal tissues during acute rejection after laryngeal transplantation in rats / 第二军医大学学报

ABSTRACT

Objective: To explore the expression of TNF-α and IL-10 in different layers of laryngeal tissues after laryngeal transplantation and its relationship with acute rejection. Methods: Laryngeal heterotopic transplantation was performed in Wistar and SD rats (Wistar→SD rats). The SD rats were divided into 4 groups: Group I: Sham control (receive no transplantation); Group II (receive transplantation, without cyelosporine A treatment); Group III (receive transplantation, with 5 mg · kg-1 · d-1 cyelasporine A treatment); and Group IV (receive transplantation, with 10 mg · kg-1 · d-1 cyelosporine A treatment). The transplanted larynx was, harvested on 3, 7 and 11 days after transplantation for pathological examination. The expression of TNF-α and IL-10 in different layers of grafts was detected immunohistochemically. Results: Pathological observation showed mild, moderate and severe acute rejection in Group II and III on 3, 7 and 11 days after transplantation, respectively; there was no obvious rejection in Group IV. Immunohistochemical staining showed expression of TNF-α and IL-10 in Group II, III, and IV, not in Group I. The ratios of the positive areas of TNF-α and IL-10 in the mucosal and submucosal layers were significantly higher than those in the muscle and cartilage layers of laryngeal tissues (P<0.01) in Group II, III, and IV. The expression levels of TNF-α in Group II, III were always significantly higher than that in Group IV (P<0.01); the expression levels of IL-10 in Group II, III were lower than that in Group IV on 11 days after transplantation (P<0.01). Conclusion: The high-antigenicity of laryngeal graft mainly locates in the mucosal and submucosal layers. Post-laryngeal transplantation expression of TNF-α and IL-10 is correlated with the course of acute rejection; detection of the expression may be used in predicting early acute rejection after laryngeal transplantation.