Correlation between immune response to hepatitis B vaccine and HLA DRB1 * 07 DRB1 * 04 DRB1 * 1001, DQB1 * 0401 genes in Han population in Ningbo / 第二军医大学学报

ABSTRACT

Objective: To study the correlation between responses to Hepatitis B and HLA DRB1 * 07 DRB1 * 04 DRB1 * 1001, DQB1 * 0401 genes in Han population in Ningbo. Methods: A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine. The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to the results: negative responses group (n = 120, anti-HBs D< 0.1) and positive response group (n = 120, anti-HBs D≥0.1). The distribution and frequency of DRB1 * 07, DRB1 * 04, DRB1 * 1001, DQB1 * 0401 genes alleles were determined with PCR using sequence-specific primers (PCR-SSP). Results: The sex and age were compatible between the 2 groups. The frequency of DRB1 * 07 in negative response group was significantly higher than that in positive response group (20.3%, vs 10.3%, P<0.05); the failure of vaccination was correlated with DRB1 * 07 (OR=0.45, P<0.05). The frequencies of HLA- DRB1 * 04 DRB1 * 1001, DQB1 * 0401 showed no obvious difference between the 2 groups. Conclusion: Failure or deficiency response to HBV vaccine in the Han people in Ningbo is associated with the HLA-DR antigen; HLA-DRB1 * 07 might be a susceptible gene for failure of HBV vaccination.