Culture and identification of canine bone marrow-derived multipotent adult progenitor cells and their differentiation into smooth muscle-like cells / 第二军医大学学报

ABSTRACT

Objective: To study the culture condition and biological characteristics of canine multipotent adult progenitor cells(MAPC) and their potential to differentiate into smooth muscle-like cells in vitro. Methods: The morphology and growth of in vitro cultured MAPC were observed and the surface marker of MAPC was detected. The cell cycle and phenotype of MAPC were determined by FACS. RT-PCR was used to examine the expression of OCT-4 in MAPC and smooth muscle cells-myosin heavy chain (SM-MHC) in the differentiated cells. The surface markers of smooth muscle cells were detected in the differentiated cells. Results: Under microscope, MAPCs were polygon and had large nuclei, multi-parapodiums and affluent cytoplasma. The cells also showed strong proliferation ability and were positive of CD13, SSEA-1 and negative of CD44, CD45, CD133, and MHC II. RT-PCR showed expression of OCT-4. The cells differentiated from MAPC expressed the surface markers of smooth muscle cells, including α-SMA, calponin and SM-MHC. RT-PCR demonstrated the expression of SM-MHC. Conclusion: We have successfully cultured canine MAPC in vitro, which has similar biological characteristics as reported previoulsy. MAPC has the potential to differentiate into smooth muscle-like cells under certain physiological condition in vitro.