Bio-oss combined with fibrin glue in reconstruction of canine mandibular defects / 第二军医大学学报

ABSTRACT

Objective: To evaluate the osteogenic activities of Bio-oss after combining with fibrin glue in reconstruction of canine mandibular defects. Methods: The second and fourth premolar teeth and the second molar teeth were extracted bilaterally, in 9 hibrid canines, resulting in 6 bone defects (1 cm × 1 cm) in each canine. Bio-oss, Bio-oss+FG and FG were implanted into the bone defects of the second, fourth premolar teeth and the second molar teeth, respectively. Canines were executed in group of 3 after 4, 8, and 12 weeks to observe the healing of soft tissues. The bone density was assessed by X-ray, the property of Bio-oss were observed via gross specimen, and the morphology of the newly-formed bone was observed by tissue sections. The proportion of newly-formed bone was obtained by computer image analysis (SAS software, analysis of variance). Results: Stage I healing of soft tissues was achieved in all animals. The bone densities were not significantly different between Bio-oss+FG and Bio-oss groups. The bone in FG group had transparent area. We also found that the bone in Bio-oss+FG group was closely combined and there were sccatered Bio-oss dusts in the soft tissues of the Bio-oss group. The newly-formed bone in the FG group was only found in the border between the defects and FG. The proportion of newly-formed bone was less in the Bio-oss+FG group than in the Bio-oss group at 4, 8, and 12 weeks after extraction(P<0.05). Conclusion: Fibrin glue can well shape the Bio-oss and has satisfactory biocompatibility, but it reduces the new bone forming.