Expression changes of brain-derived neurotrophic factor in hippocampus of rats with post-epilepsy learning and memory deficiency / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 488-491, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-841127
ABSTRACT
Objective:
To study the relationship between the learning/memory ability and the expression changes of brain-derived neurotrophic factor (BDNF) mRNA and protein in hippocampus of epileptic rats kindled by Pentylenetetrazole (PTZ).Methods:
Adult male Sprague-Dawley (SD) rats, weighing (200 ± 20) g, were randomly divided into status epilepticus (SE) group, Control I, chronic epilepsy (CEP) group and Control II. Rats in SE group were initially administrated i. p. with 40 mg/kg PTZ, 10 min later with 20 mg/ kg PTZ, and then with 10 mg/kg PTZ thereafter until the development of SE; rats in CEP group were administrated with 35 mg/kg PTZ for 3 times at an interval of 48 h, until the development of Racine IV-V epilepsy; and rats in Control I and Control II groups were given normal saline in the same manners as SE group and CEP group, respectively. Alternative electro-stimulus Y-maze test was used to evaluate the learning/memory ability of rats; RT-PCR and immunohistochemistry method were used to determine the expression of BDNF mRNA and protein in rats' hippocampus.Results:
On the first and second day after kindling, we found that the total errors of rats in SE group were obviously increased compared with those in Control I (P<0.05); the total errors of CEP group on the first, thirtieth and thirty-first day were obviously increased compared with those in Control II (P<0.05). Lower expression of BDNF mRNA and protein in hippocampus was found 1 day after kindling in SE and CEP group and 30 days after kindling in CEP group.Conclusion:
Status epilepsy can induce short term deficiency of learning and memory in rats, while chronic epilepsy induces a longer deficiency. Learning and memory deficiency caused by epilepsy might be related to the decrease of BDNF mRNA and protein expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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