Protective effect of Er-xian decoction on ovariectomy-induced osteoporosis in rats / 第二军医大学学报

ABSTRACT

Objective: To investigate the protective effects of Er-xian decoction on ovariectomy-induced osteoporosis in rats. Methods: The osteoporosis model was induced by ovariectomy in rats. Twelve weeks later, the undecalcified longitudinal proximal tibial metaphysical sections were made and stained for the bone histomorphometric analysis. Bone density of femur metaphysis was determined by dual-energy X-ray absorptiometry. Results: Er-xian decoction significantly increased trabecular area and trabecular thickness (P<0.01) and decreased trabecular separation (P<0.05). The parameters of bone formation of rats, such as MAR, BFR /BS, BFR/BV, and BFR/TV, were increased significantly after Er-xian decoction treatment (P< 0.05, P<0.01). Moreover, Er-xian decoction obviously reduced osteoclast number (P<0.01) and enhanced bone density of femur metaphysis in ovariectomized rats (P < 0.05). Conclusion: Er-xian decoction has antiosteoporotic effects on ovariectomized rats by promoting bone formation, increasing bone density, and inhibiting bone resorption.