Shanghai multi-organ solution in preservation of rat liver: An experimental study / 第二军医大学学报

ABSTRACT

Objective: To observe the preservative effect of Shanghai-mutil-organ (SMO) solution on rat liver and to assess the feasibility of SMO solution in preserving isolated human livers. Methods: SD rats were randomly divided into 3 groups according to the preservation solutions SMO group, UW ( University of Wisconsin Solution) group, and HTK (Histidinetryptophan-ketoglutarate solution) group. The simple cold storage liver model was established with isolated rat liver and the liver samples from each group were preserved with the 3 solutions for 8 h, 16 h, 24 h and 36 h. The energy metabolism was analyzed in all the samples and the morphological changes and hepatocytes apoptosis were observed after different preservation periods. Results: The liver tissue contents of ATP, TAN, and AEC in SMO group were significantly higher than those in HTK group at 16 h, 24 h, and 36 h (P<0.05); the contents in SMO group were similar to those in UW group. Histological examination showed that the tissue damage in SMO group was milder than that in HTK group; the damages in SMO group and UW group were similar except that the swelling of cells in SMO group was severer than that in UW group. Apoptosis index in SMO group was lower than that in HTK group at 24 h and 36 h(P< 0.05); there was no significant difference in apoptosis index between SMO group and UW group. Conclusion: SMO solution has a similar preservative effect on rat liver to that of UW solution, only with more severe cell swelling than the latter. The effect of SMO solution is better than that of HTK solution.