Modification of catalyzed signal amplification method / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 209-211, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-841209
ABSTRACT
Objective:
To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification (CSA).Methods:
Using 7 different types of primary antibodies and 2 tumor microarray system, we carried out En Vision, LSAB, Standard CSA, and modified CSA staining and compared their sensitivities and specificities.Results:
The modified CSA method was the most sensitive one among the 4 methods, followed by standard CSA, En Vision, and LsAB method. The sensitivity of modified CSA method was 2-3 times higher than that of standard CSA. The 4 methods had similar background staining and had exact localization of cells.Conclusion:
The modified CSA is more stable and sensitive than traditional CSA staining method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2010
Type:
Article
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