Effects of chitosan and PC-chitosan-coated membranes on growth and hemocompatibility of vascular endothelial cells / 第二军医大学学报

ABSTRACT

Objective: To investigate the effects of chitosan (CHI) and PC-chitosan (PC CHI) coated membranes on the proliferation, migration, and hemocompatibility of vascular endothelial cells. Methods: CHI and PC-CHI were separately sprayed on the bottom of culture dish evenly to form the polymer membrane; the cylinder was made of 316 L stainless steel flake. Pig iliac endothelial cells (PIEC) were cultured on the polymer membranes, the stainless steel bottom of the cylinder, and the untreated bottom of culture dish (as blank control). Then the cell morphology was observed by light microscope and scan electron microscope; cell proliferation was measured by Cell Counting Kit-8 (CCK 8); and cell migration was also assayed. Whole blood activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FiB), and thrombin time (TT) were examined by clotting method. Results: PIEC grew well on the membranes of CHI and PC-CHI, with normal morphology. After 24 h culture, the proliferation rates of PIEC were 88. 8% and 77. 8% on CHI and PC-CHI membranes, respectively. The survived PIEC on CHI and PC-CHI membranes were significantly more than those on 316 L stainless steel flake(P<0.01), and those on CHI membrane were significantly more than those on PC-CHI membrane(P<0.01). After 72 h culture, the numbers of migration cells on CHI and PC-CHI membranes were significantly more than those on 316 L stainless steel flake(P<0.01), and those on PC-CHI membrane were significantly more than those on CHI membrane(P<0.01). APTT in PC-CHI, 316 L stainless steel flake, and blank control groups were significantly longer than that of CHI group(P<0.01, P<0.05), and FiB levels in the former 3 groups were higher than that of CHI group(P<0.01). Conclusion: Coating with CHI and PC-CHI can improve the proliferation and migration of vascular endothelial cells on the culture dishes, indicating an excellent biocompatibility of CHI and PC-CHI membranes. PCPCH membrane has more potent antithrombotic effect than CHI does.