Isolation and purification of water-soluble polysaccharide MP-I from Mytilus coruscus and study on its in vitro anti-tumor activity / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 998-1001, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-841332
ABSTRACT
Objective:
To separate and purify polysaccharide MP- I from Mytilus coruscus and to investigate its in vitro anti-tumor activity.Methods:
The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method. The polysaccharide MP- I was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography. Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography. The in vitro anti-tumor activity of MP- I was assessed using MTT method.Results:
The yield of MP- I was 2.14%. MP- I is mainly constituted by glucose. MP- I (at 0.5, 0.1, 0.02 mg/ml) had different degrees of inhibitory effects on HO-8910, MCF-7, K562, and SMMC-7721 tumor cells in vitro (P<0.01). The inhibitory rates of MP- I at 0. 5 mg/ml against HO-8910 and MCF-7 cells were 30.55% and 36.38%, respectively.Conclusion:
Polysaccharide MP- I is mainly constituted by glucose and it has inhibitory effect on tumor cell lines HO-8910, MCF-7, K562, and SMMC-7721 in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2006
Type:
Article
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