Expression of zinc finger protein 262 mRNA in patients with autosomal-dominant polycystic kidney disease and its significance / 第二军医大学学报

ABSTRACT

Objective: To investigate the expression of zinc finger protein 262 (ZNF262) mRNA in normal kidney tissues and kidney tissues of patients with autosomal-dominant polycystic kidney disease (ADPKD) at different stages, and to explore the role of ZNF262 in pathogenesis of ADPKD. Methods: Patients with ADPKD were staged according to glomerular filter rate (GFR). Imaging observation and routine pathological examination were performed. The expression of ZNF262 mRNA and proliferating cell nuclear antigen (PCNA) mRNA in normal kidney tissues (n=8), early stage ADPKD kidney tissues (n=4) and advanced stage ADPKD kidney tissues (n=4) was examined by semi-quantitative RT-PCR. The correlation between the expressions of the 2 genes was investigated in all tissue specimens. Results: Expression of ZNF262 mRNA and PCNA mRNA in early and advanced ADPKD kidney tissues was significantly higher than that in normal renal tissues (both P<0.01), and that in the advanced stage ADPKD was significantly higher than that in early stage ADPKD (both P<0.05). The expression of ZNF262 and PCNA mRNA was highly correlated in the early, advanced ADPKD and normal renal tissues(r1 =0.842 6, r2=0.902 1 and r3=0.883 5, respectively, all P<0.05). Conclusion: The ZNF262 mRNA lev el is higher in ADPKD kidney tissue than that in normal control and increases with the advancement of ADPKD. The expression of ZNF262 is significantly correlated with the expression of PCNA in the same renal tissues. The expression of ZNF262 mRNA may serve as an indicator in diagnosis of ADPKD and may be used for clinical staging of ADPKD patients.