In vitro evaluation of biocompatibility and surface modification of 3 kinds of artificial graft material / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 400-404, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-841447
ABSTRACT
Objective:
To evaluate the biocompatibility of 3 kinds of artificial, biodegradable graft materials, including T20 [Poly (butylene terephthalate)-co-poly (cyclohexylene dimethanol terephthalate)-b-poly (ethylene glycol)], PHBV (Poly3-hydroxybutyrate-Poly3-hydroxyvalerate) and PEGT/PBT [Poly (butylene terephthalate)/poly (ethylene glycol) terephthalate], and to study their effects on canine smooth muscle cells(SMCs) growth after surface modification.Methods:
The contact angles were measured to compare the hydrophilicity of T20, PHBV, and PEGT/PBT scaffolds. Then SMCs were cultivated on these biodegradable scaffolds; their proliferation and growth were assayed by MTT assay, FITC and electron microscopic observation for evaluation of their in vitro biocompatibility. The growth of SMCs was also assayed on the 3 scaffolds after they were pretreated with gelatin or Poly-1-lysine.Results:
The contact angles of 3 scaffolds were all less than 90°(PEGT/PBT>T20>PHBV). MTT assay showed that SMCs adhered to and grew well on T20, PHBV, and PEGT/PBT scaffolds. The cell viability began to increase 24 h after cultivation (PEGT/PBT>T20>PHBV, PPEGT/PBT>PHBV, P<0.01). FITC observation showed that SMCs grew well and electron microscopic observation showed a confluent growth of SMCs with abundant extracellular matrix. The viability of SMCs was significantly higher on scaffolds pretreated with gelatin or Poly-1-lysine than on those without pretreatment(P<0.01).Conclusion:
T20, PHBV, PEGT/PBT have good in vitro biocompatibility; the scaffolds made of these materials can obviously improve cell attachment after surface modification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2006
Type:
Article
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