Protective effects of adenovirus-mediated gene transfer of soluble complement receptor type 1 on acute myocardium ischemia: An experimental study / 第二军医大学学报

ABSTRACT

Objective: To evaluate the protective effects of adenovirus-mediated gene transfer of soluble complement receptor type 1 (sCR1) on acute myocardium ischemia in mice. Methods: Twenty-seven SD rats were subjected to left anterior descending coronary artery (LAD) occlusion (30 min) and reperfusion. In the treatment group (n = 14), a mixture of adenovirus carrying sCR1 and LacZ was injected into the ischemic zone(100 μl, 1010 pfu) 5 min before reperfusion; in the control group, only adenovirus carrying LacZ was injected (n = 13). Echocardiography was performed 2 weeks later, followed immediately by pathologic examination. Results: Echocardiographic results of the treatment group were better than those of the control group (P<0.05) 2 weeks after the operation, especially when it comes to the size of infarction area (P<0.01). H-E staining showed more surviving myocardial cells in the treatment group than in the control group. Conclusion: Transfer of sCR1 gene by adenovirus can reduce the size of infarction area and preserve heart function in a long-term period.