Differential gene expression pattern between autosomal dominant polycystic kidney tissue and normal kidney tissue: A DNA microarray study / 第二军医大学学报

ABSTRACT

Objective: To study the differential gene expression pattern between autosomal dominant polycystic and normal kidney tissue, and to deduce the etiological factor and treatment for autosomal dominant polycystic kidney disease (ADPKD). Methods: The cDNA probes were prepared by label ling normal kidney tissue mRNA and ADPKD tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The probes were then hybridized with the DNA microarrays (PCR products of 4 096 human cDNAs onto specially treated glass slides) and the fluorescent signals were scanned with ScanArray 4000 scanner. Semi-quantitive RT-PCR was performed to test the expression level of 4 related genes. Results: Of the 4096 genes screened, 463 genes showed obvious changes. Expression of 206 genes was upregulated in the polycystic kidney tissue, especially cyclin D2, MMPs, TIMP1 and fibroblast activation protein; expression of 257 genes was downregulated, especially phosphatase 1A and acid phosphatase 1. The expression of genes tested by RT-PCR was in accordance with those detected by cDNA microarray. Conclusion: ADPKD may be related to the upregulation of cyclin,MMPs,and various kinds of growth factors, and drugs like inhibitors of CaM and MMPs might have therapeutical effects on ADPKD.