Differential gene expression pattern between autosomal dominant polycystic kidney tissue and normal kidney tissue: A DNA microarray study / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 182-185, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-841500
ABSTRACT
Objective:
To study the differential gene expression pattern between autosomal dominant polycystic and normal kidney tissue, and to deduce the etiological factor and treatment for autosomal dominant polycystic kidney disease (ADPKD).Methods:
The cDNA probes were prepared by label ling normal kidney tissue mRNA and ADPKD tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The probes were then hybridized with the DNA microarrays (PCR products of 4 096 human cDNAs onto specially treated glass slides) and the fluorescent signals were scanned with ScanArray 4000 scanner. Semi-quantitive RT-PCR was performed to test the expression level of 4 related genes.Results:
Of the 4096 genes screened, 463 genes showed obvious changes. Expression of 206 genes was upregulated in the polycystic kidney tissue, especially cyclin D2, MMPs, TIMP1 and fibroblast activation protein; expression of 257 genes was downregulated, especially phosphatase 1A and acid phosphatase 1. The expression of genes tested by RT-PCR was in accordance with those detected by cDNA microarray.Conclusion:
ADPKD may be related to the upregulation of cyclin,MMPs,and various kinds of growth factors, and drugs like inhibitors of CaM and MMPs might have therapeutical effects on ADPKD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2006
Type:
Article
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