Bioactive cyclodipeptides extracted from marine microbes in East China Sea / 第二军医大学学报

ABSTRACT

Objective: To search for bioactive compounds from marine microbes. Methods: Pyricularia oryzae was used to screen microbes. Compounds extracted with EtOAc from the fermentation broth of F8712 strain(an active microbe screened out) were separated. The chemical structures of the separated compounds were identified by HNMR, CNMR and ESI-MS techniques. Results: Six cyclic dipeptides, cyclo(Ala-Leu), cyclo(Ala-Ile), cyclo(Ile-Pro), cyclo(Val-Pro), cyclo(Leu-Pro) and cyclo (Leu-Val), were obtained. Conclusion: None of the 6 cyclodipeptides has any anti-Pyricularia oryzae activities. Compound V has potent inhibitory effects on Vibrio anguillarm with a minimum inhibitory concentration of 0.07 μg/ml.