Effect of medroxyprogesterone acetate on proliferation and apoptosis of human colorectal cancer cells / 第二军医大学学报

ABSTRACT

Objective: To investigate the effects of medroxyprogesterone acetate (MPA) on the proliferation and apoptosis of human colorectal cancer cells. Methods: Human colorectal cancer cell line LS174T was treated with different concentrations of MPA(12.5,25,50 and 100 μmol/L ) for 120 h. The inhibitory effect of MPA was measured with MTT method; the cell cycle, cell apoptosis, and cell surface Fas expression were analyzed with flow cytometry. Results: MPA had significant inhibitory effect on the growth of LS174T human colon cancer cells. Cell growth was found to be arrested in G1 phase and cells in S phase and G2/M phase were decreased. The natural apoptosis rate and the apoptosis rates at MPA 12.5 and 25 μmol/L were 4.8%, 15.1% and 25.6%, respectively. Cell surface Fas expression in cancer cells increased after treated with MPA. Conclusion: MPA has significant inhibitory effect on the growth of human colon cancer cells, which may be related to Fas expression upregulation on cancer cell surface and G1 phase arrest.