Antiaddictive indole alkaloids in Ervatamia yunnanensis and their bioactivity / 第二军医大学学报

ABSTRACT

Objective: To investigate the constituents of indole alkaloids in Ervatamia yunnanensis and their antiaddictive bioactivities. Methods: The preliminary alkaloids (TEYA) were extracted by 95 % EtOH from the stems of Ervatamia yunnanensis. TEYA was then purified by silica gel and Sephadex LH20 column chromatography repeatedly and its structure was identified with spectral analysis. The antiaddictive effects of these indole alkaloids were evaluated by Conditioned Place Preference (CPP) experiments. Results: Nine indole alkaloids were obtained and identified as coronaridine (1) , voacangine (2), 3-R-ethoxycoronaridine (3), 3-S-ethoxycoronaridine (4), 19-epi-heyneanine (5), heyneanine (6), 19-epi-voacristine (7), coronaridine hydroxyindolenine (8) and 12-methoxyl-voaphylline (9). Among them voacangine (2) and 19-epi-voacristine (7) were effective in prevention and treatment of addiction induced by morphine in rats (n=8). Conclusion: Compounds 3, 4, 8, and 9 have been isolated from Ervatamia yunnanensis for the first time and coronaridine-type indole alkaloids are the main antiaddictive constituents of E. yunnanensis.