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Effect of schisandra chinensis polysaccharide on serum inflammatory factors in T2DM rats and its mechanism / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition) ; (6): 50-55, 2020.
Article in Chinese | WPRIM | ID: wpr-841580
ABSTRACT

Objective:

To observe the effect of Schisandra Chinensis polysaccharide (SCP) on the serum inflammatory factors in the rats with type 2 diabetes mellitus (T2DM) induced by high-fat diet and low dose of streptozotocin (STZ), and to explore its underlying mechanism in the treatment of T2DM.

Methods:

The male Wistar rats were given high-fat diet and introperieoneally injected with low dose of STZ (30 mg · kg-1) in one time to establish the rat T2DM models. The successful model rats were randomly divided into model group, low dose (25 mg · kg-1)of SCP group, middle dose (50 mg · kg-1) of SCP group and high dose (100 mg · kg-1) of SCP group; there were 10 rats in each group. Another 10 healthy rats were used as normal control group. Eight weeks after the intragastric administration of SCP, oral glucose tolerance test (OGTT) was performed in the rats of various groups. The fasting blood glucose (FBG) and insulin (INS) levels were detected by glucose oxidase method and radioimmunoassay method, respectively, and the insulin sensitivity index (ISI) was calculated. The levels of interleukin-6 (IL-6), C-reactive protein (CRP), interleukin-lβ (IL-lβ), tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in serum of the rats were measured by enzyme linked immunosorbent assay (ELISA) method. HE staining was used to observe the pathomorphology of pancreas tissue of the rats.

Results:

Compared with normal control group, the serum FBG level of the rats in model group was significantly increased (P<0.01), and the area under the curve (AUC) of blood glucose of the rats was significantly increased, the serum INS level and the ISI were significantly decreased (P<0.05 or P<0.01); the levels of IL-6, CRP, IL-1β, TNF-α, and NF-κB in serum were all significantly increased (P<0.05 or P<0.01). Compared with model group, the serum FBG levels of the rats in different doses of SCP groups were markedly decreased (P<0.05), the AUC of blood glucose of the rats were significantly decreased, the INS and the ISI levels were significantly elevated (P<0.05); the levels of IL-6, CRP, IL-1β, TNF-α and NF-κB in serum were significantly decreased (P<0.05 or P<0.01). Compared with low dose of SCP group, the FBG levels of the rats in middle and high doses of SCP groups were significantly decreased (P<0.05), the serum INS level of the rats in high dose of SCP group was significantly increased (P<0.05). The HE staining results showed that compared with normal control group, the islets were atrophied, the number of cells in islets was decreased and the boundary was irregular in model group; compared with model group, the islet boundary in different doses of SCP groups became clear, the areas were increased, and the number cells was increased significantly.

Conclusion:

SCP can decrease the FBG level, increase the INS level and improve insulin resistance (IR) in the T2DM rats induced by high-fat diet combined with low dose of STZ, and its mechanism might be related to its inhibiting inflammation response.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2020 Type: Article