Effects of brd4 gene on apoptosis and p38mapk signaling pathway of cardiomyocytes induced by tgf-pi / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effect of bromodomain-containing protein 4 (BRD4) gene on the apoptosis of cardiomytransforming growth factor-,31 (TGF-,31). and to clarify its possible mechanism.

Methods:

The II9c2 cells were randomly divided into blank control group. si-CN group (transfected with negative control siRNA) . and si BRD4 group (transfected with specific siRNA). The expression levels of BRW protein in the cells in various groups were detected by Western blotting method. The Il9c2 cells were randomly divided into control group. TGF-,31 group (transfected with 20 [xg • L" TGF-,31 for 24 h) . si-NC+TGF-,31 group (treated with TGF-,31 for 24 h after transfected with negative control siRNA) and si-BRD4 +TGF-,31 group (treated with TGF-,31 for 24 h after transfected with BRD4 specific siRNA). The cell proliferation activity was measured by MTT assay, and the apoptotic rate of cells was detected by Annexin V-FITC/PI double staining. Western blotting method was used to detect the expressions of BRD4. Bcl-2» Bax∗ Cleaved caspase 3∗ P38» and p-P38 proteins.

Results:

Compared with blank control group, the expression level of BRD4 protein in the Il9c2 cells in si-BRD4 group was significantly decreased (P<0. 01). Compared with control group, the cell proferation activity and the Bcl-2 protein expression level in TGF-,31 group were significantly decreased ( Pproteins were significantly increased (Pcell prdiferation activity and the expression level of Bcl-2 protein in si BRD4 + TGF-pl group were significantly increased (PConclusion: Inhibition of BRD4 gene expression can attenuate the apoptosis of cardiomyocytes induced by TGF-