Expression of tgf-|βl in scleral fibroblasts of rats with form deprivation myopia and regulation of wnt/β-catenin signaling pathway / 吉林大学学报(医学版)

ABSTRACT

Objective: To construct the form deprivation myopia (FDM) rat models, and to elucidate the expression of transforming growth fact or-(21 CTGF-ß1 ) in scleral fibroblasts of the FDM rats and its relationship with Wnt/p-catenin signaling pathway. Methods: Forty rats were randomly divided into control group and FDM model group, with 20 rats in each group. The FDM rat model was established in FDM model group. The axial lengths of the rats were determined. The rat sclera tissue of eyeball was separated. The expression levels of TGF-ß1 protein and mRNA in sclera tissue of the rats were determined by Western blotting and reverse transcription-polymerase chain reaction CRT-PCR) methods. The scleral fibroblasts of rats were isolated and cultured. The fibroblasts in the sclera of the rats in control group were used as the control group. The fibroblasts in FDM model group were divided into FDM group and FDM+ Dickkopf related protein 1 (DDK1) group (added to DDK 1 to culture). The expression levels of TGF-ß1. Dicer-like 3 (DCL3)» colon adenomatous polyp protein CAPO, glycogen synthase kinase 30 (GSK3{3)» p21-GSK3j3. and {3-catenin protein and mRNA in scleral fibroblasts were determined by Western blotting and RT-PCR methods. Results: Compared with control group-the axial length of the rats in FDM group was increased (P0. 05) ; but there were significant differences in the expression levels of TGF-ß1, DC 1.3. APC. p21-GSK30. and p-catenin protein and mRNA in the scleral fibroblasts C P< 0.01). Compared with control group, the expression levels of TGF-ß1 and APC protein and mRNA in scleral fibroblasts of the rats in FDM group were decreased (P<.0. 01). and the expression levels of DCL3» p21-GSK3(3. and (3-catenin protein and mRNA were increased ( P<0. 05). Compared with FDM group, the expression levels of TGF-ß1 and APC protein and mRNA in the scleral fibroblasts of the rats in FDM+DDK1 group were increased CP<0. 01). and the expression levels of DCL3. p21-GSK3,3. and 0-catenin protein and mRNA were decreased ( P< 0. 01). Conclusion: The expression level of TGF-,ß1 in the scleral fibroblasts of the FDM model rats is decreased, and its level is regulated by the Wnt. p-catenin signaling pathway.