Expression of SIRT6 protein in colon cancer tissue detected by tissue microarray technique and its clinical significance / 吉林大学学报(医学版)

ABSTRACT

Objective:To investigate the expression of NAD-dependent protein deacetylase sirtuin-6(SIRT6) protein in the colon cancer tissue and the association between its expression and the clinicopathological parameters of the patients with colon cancer, and to elucidate the effect of the SIRT6 protein in the occurrence and development of colon cancer.Methods:The colon cancer tissue and the paracancerous tissue obtained from 100 patients who did not receive radiotherapy and chemotherapy before operation were selected.The expression levels SIRT6 protein in colon cancer tissue and paracancerous tissue were detected by tissue microarray technique and immunohistochemical SP method. The relationships between the expression of SIRT6 and the clinicopathological parameters of the patents with colon cancer were analyzed with rank sum test. Kaplan-Meier survival curre,univariate analysis and multivariate survival analysis were used to investigate the relationships between the clinicopathological parameters and the prognosis in the patients with colon cancer.Results:The expression levels of SIRT6 in cytoplasm and nucleus in colon cancer tissue were significantly higher than those in paracancerous tissue (Z=-4.603,P=0.000;Z=-7.610,P=0.000).The Kaplan-Meier univariate and multivariate analysis showed that the prognosis of the patients with high expression of SIRT6 in the nucleus was better, and the expression of SIRT6 was identified as the independent prognostic factor of colon cancer(HR=2.345,P=0.003); the patients with younger age, lower T and N stages had higher survival rates (HR=0.394,P=0.004; HR=2.301,P=0.012; HR=2.423,P=0.048);there was no correlation between the high expression of SIRT6 in cytoplasm and the prognosis (HR=1.309,P=0.333) of the patients with colon cancer.Conclusion:SIRT6 protein is highly expressed in the nucleus of colon cancer and it can be used as a good prognostic indicator for colon cancer.