Effects of atorvastatin on expression of lipid metabolism- related genes in HepG2 cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To explore the effects of atorvastatin on the natural antisense transcription of apolipoprotein A1 (apoAl-NAT) in the HepG cells and its influence in the expressions of lipid metabolism related genes. Methods: The HepG cells were intervened with different concentrations (0, 1, 10 and 100 nmol • L-1 ) of atorvastatin, and the 0 nmol • L-1 atorvastatin group was used as control group. The total RNA of HepG cells in various groups were extracted at different time points (6, 12, 24, and 48 h). The mRNA expression levels of lipid metabolism-related genes and apoAl-NAT expression levels were detected by Real-Time PCR method. Results: The morphology of HepG cells in 1 and 10 nmol • L-1 atorvastain groups was normal. Compared with 6 h, the expression levels of apoAl-NAT in the HepG cells in 10 nmol • L atorvastatin group at 12, 24 and 48 h were significantly decreased (Ptime-dependent manner. Under the same condition, the expression level of apoAl mRNA in the HepG cells at 48 h was increased significantly compared with 6 h ( P<0.01). Compared with control group, the expression levels of low density lipoprotein receptor (LDLR) in the HepG cells in 100 nmol • L atorvastatin group, scavenger receptor-class B type 1 (SRB1) in 10 nmol • L-1 atorvastatin group and ATP binding cassette transporter A1 (ABCA1) in 1 and 10 nmol • L atorvastatin groups were increased (P'<0. 01) in different degrees.

Conclusion:

Atorvastatin can promote the expressions of lipid metabolism-related genes by inhibiting the expression of apoAl-NAT, and promote the process of reverse cholesterol transport.